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  4. 6*His Monoclonal Antibody

6*His Monoclonal Antibody

文献引用 (3) Datasheet
  • 货号：
    CSB-MA000011M0m
  • 规格：
    ￥600
  • 图片：

    • Western Blot
      Positive WB detected in: HIS fusion protein 1 at 20ng, 10ng, 5ng, 2.5ng
      All lanes: HIS antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 55 kDa
      Observed band size: 55 kDa
       

    • Western Blot
      Positive WB detected in: HIS fusion protein 2 at 20ng, 10ng, 5ng, 2.5ng
      All lanes: HIS antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 60 kDa
      Observed band size: 60 kDa

  • 其他：

产品详情

  • 产品描述：

    The 6His Monoclonal Antibody specifically reacts to recombinant proteins containing the 6xHis-Tag. The 6*His tag is widely used in recombinant protein isolation and purification, and this antibody is a valuable tool for researchers who need to detect or isolate proteins that have been tagged with the 6*His sequence. This antibody is immunized with a 6*His synthetic peptide conjugated to KLH and produced in mouse.

    The 6*His Monoclonal Antibody underwent protein A mediated purification and was purified to a high degree of purity, >95%. It is available in liquid form and belongs to the IgG2a isotype. This antibody has been validated in both ELISA and WB assays, making it a useful tool for the detection and quantification of recombinant proteins containing the 6*His-Tag. Its high specificity and purity ensure reliable and reproducible results. This antibody has been cited in a paper by Dominguez G.et al. in 2018, increasing its reliability and usefulness.

  • 别名：
    6*His, 6*His, His Tag, 6histag, 6*His, HHHHHH, HIS, His Tag
  • 宿主：
    Mouse
  • 反应种属：
    N/A
  • 免疫原：
    HHHHHH(6x His) synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    3G5H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
    ELISA Protocol
    Western Blotting (WB) Protocol
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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