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  4. Human telomerase,TE ELISA Kit

Human telomerase,TE ELISA Kit

文献引用 (7) 评价 (1) 说明书下载
  • 中文名称：
    人端粒酶(TE)酶联免疫试剂盒
  • 货号：
    CSB-E08021h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human telomerase (TE) ELISA Kit was designed for the quantitative measurement of Human telomerase (TE) protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    N/A
  • 缩写：
    TE
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, cell culture supernates, urine, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human TE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-96
    1:2Average %99
    Range %91-105
    1:4Average %94
    Range %92-98
    1:8Average %89
    Range %86-100
  • 回收率：
    The recovery of human TE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8980-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.630 2.501 2.566 2.464
    102.161 2.062 2.112 2.010
    51.602 1.541 1.572 1.470
    2.50.931 0.898 0.915 0.813
    1.250.532 0.501 0.517 0.415
    0.6250.322 0.314 0.318 0.216
    0.3120.241 0.227 0.234 0.132
    00.105 0.099 0.102 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：蛋白（重组/纯化） 细胞裂解后的胞浆蛋白

样品信息：人

稀释比：其他 1:2

产品评价： 我用CSB-E08021h测量癌细胞裂解液中的端粒酶含量，稀释一倍，OD在0.3左右。标曲正常，端粒酶定量也符合文献报道。使用方便，如果可以的话盒子做小一点，更好保存。

By 黄老师

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问答及客户评论

■ 常见问题解答
Q:

We are interesting in knowing the composition and the origin of the standard as well as capture and biotin antibody in this kit.

A:
Thanks for your inquiry.
Coated antibody: Mouse monoclonal antibody
Detect antibody: Goat polyclonal antibody
Standard: It is prepared with a special method and sorry we cannot provide the detailed information. Hope you could understand.
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