Mouse interleukin-18 binding prorein(IL-18BP) ELISA Kit

产品详情

文献引用 (1) 评价 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Mouse IL-18BP ELISA Kit was designed for the quantitative measurement of Mouse IL-18BP protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名：

Il18bp ELISA Kit; Igifbp ELISA Kit; Interleukin-18-binding protein ELISA Kit; IL-18BP ELISA Kit; Interferon gamma-inducing factor-binding protein ELISA Kit
缩写：

IL-18BP
Uniprot No.：

Q9Z0M9
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	84
	Range %	80-88
1:4	Average %	104
	Range %	100-107
1:8	Average %	91
	Range %	87-95

回收率：

The recovery of mouse IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	89	84-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 个评价

样品类型：组织匀浆液小鼠结肠

样品信息：小鼠

稀释比：其他稀释2倍

产品评价：我用了货号为CSB-E17797m的Mouse Interleukin-18 Binding Prorein (IL-18BP)ELISA Kit检测了DSS诱导结肠炎之后小鼠结肠组织匀浆中的IL-18BP蛋白，OD值为2左右，该试剂盒效果不错，反应灵敏，达到了预期实验的目的

By 胡老师

功能：

Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
基因功能参考文献：
1. IL-18BP ameliorates cardiac ischemia/reperfusion injury in part through suppression of Th17 differentiation. PMID: 26340315
2. Data show that IL-18bp acts as a sensor for IFN-gamma and can regulate both Th1 and Th17 responses in the CNS. PMID: 20644165
3. Administratioan of murine IL-18BP at the time of the booster injection of bovine CII reduces the progressive severity of collagen-induced arthritis; both clinical disease activity and histological scores are decreased by 50%. PMID: 12574381
4. functional polarization of Ag-specific CD4(+) Th2 cells entering the CNS during the accelerating phase of experimental autoimmune encephalomyelitis is flexible and dependent on IL-18, which is overturned by IL-18 binding protein PMID: 15778395
5. IL-18 and IL-18BP may have roles in testicular development, and also in the regulation of testicular functions under physiological conditions PMID: 18299271
6. A single intravenous dose of recombinant mouse IL18BP decreased neointimal hyperplasia and improved arterial L/A ratios in an atherosclerotic balloon-injury animal model. PMID: 18455646
显示更多

收起更多
亚细胞定位：

Secreted.
数据库链接：

KEGG: mmu:16068

STRING: 10090.ENSMUSP00000091685

UniGene: Mm.422756