Rat α2 macroglobulin,α2 MG ELISA Kit

文献引用 (1) 说明书下载

产品详情

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	88-105
1:2000	Average %	91
	Range %	89-102
1:4000	Average %	93
	Range %	90-98
1:8000	Average %	94
	Range %	87-98

回收率：

The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
亚细胞定位：

Secreted.
蛋白家族：

Protease inhibitor I39 (alpha-2-macroglobulin) family
组织特异性：

Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
数据库链接：

KEGG: rno:100911545

STRING: 10116.ENSRNOP00000019346

UniGene: Rn.225884