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  4. Human 5-Hydroxytryptamine,5HT ELISA Kit

Human 5-Hydroxytryptamine,5HT ELISA Kit

文献引用 (2) 说明书下载
  • 中文名称：
    人5羟色胺(5-HT)酶联免疫试剂盒
  • 货号：
    CSB-E08363h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human 5HT ELISA Kit was designed for the quantitative measurement of Human 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 4 ng/mL-800 ng/mL and the sensitivity is 2 ng/mL.

  • 缩写：
    5HT
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    4 ng/mL-800 ng/mL
  • 灵敏度：
    2 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-102  
    1:2 Average % 94  
    Range % 91-98  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 95  
    Range % 89-99  
  • 回收率：
    The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 89 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    800 0.215 0.216 0.216    
    200 0.383 0.391 0.387    
    64 0.575 0.567 0.571    
    16 0.986 0.974 0.980    
    4 1.571 1.472 1.522    
    0 2.444 2.345 2.395    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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