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  4. Rat 5-Hydroxytryptamine,5HT ELISA Kit

Rat 5-Hydroxytryptamine,5HT ELISA Kit

文献引用 (22) 说明书下载
  • 中文名称：
    大鼠5羟色胺(5-HT)酶联免疫试剂盒
  • 货号：
    CSB-E08364r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
        血清素，也称为 5-羟色胺 (5HT)，是大脑中一种重要的神经递质，参与情绪调节、睡眠和食欲。它是人体奖励系统的重要组成部分，有助于调节人的情绪状态。 5HT 在抑郁症、焦虑症和其他心理健康障碍中起着关键作用。此外，它还与各种身体疾病有关，例如偏头痛和肠易激综合症。
        华美生物所提供的Rat 5-Hydroxytryptamine,5HT ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测大鼠血清、血浆、细胞裂解液、组织匀浆样本中的5HT，其灵敏度为华美生物所提供的0.4 ng/mL，检测范围为0.8 ng/mL-160 ng/mL。
     
  • 缩写：
    5HT
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围：
    0.8 ng/mL-160 ng/mL
  • 灵敏度：
    0.4 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-98  
    1:2 Average % 95  
    Range % 92-99  
    1:4 Average % 97  
    Range % 89-103  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    160 0.069 0.074 0.072    
    40 0.181 0.195 0.188    
    12.8 0.426 0.439 0.433    
    3.2 0.802 0.855 0.829    
    0.8 1.155 1.231 1.193    
    0 2.158 2.227 2.193    
  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit 5HT antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-rat 5HT Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated 5HT 1 x 6 ml -- Bind to the 5HT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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靶点详情

  • 最新研究进展：
        5-羟色胺 (5-hydroxytryptamine, 5HT) 是一种神经递质，参与多种生理和行为过程。以下是最近一些关于5HT的研究进展：
        5HT在抑郁症中的作用：5HT被认为是调节情绪的重要神经递质之一。最近的研究表明，抑郁症患者的5HT水平通常较低。因此，提高5HT水平可能是一种有效的治疗抑郁症的方法。最近的临床研究表明，与传统抗抑郁药物相比，一些新型的5HT受体激动剂能够更有效地改善抑郁症状。
        5HT与自闭症的关系：最近的研究表明，5HT可能与自闭症的发生有关。研究人员发现，自闭症患者的5HT水平通常较低，并且5HT受体基因突变可能与自闭症的发生有关。因此，通过增加5HT水平或改善5HT信号通路可能成为一种治疗自闭症的方法。
        5HT在肠道功能障碍中的作用：5HT在肠道中也起着重要作用，参与肠道平滑肌收缩和分泌调节。最近的研究表明，5HT信号通路的紊乱可能与肠道功能障碍有关。一些新型的5HT受体激动剂已被用于治疗肠道功能障碍，但其长期疗效和安全性还需要进一步的研究。
        5HT在神经退行性疾病中的作用：最近的研究表明，5HT可能在神经退行性疾病的发生和进展中发挥作用。研究人员发现，5HT受体在阿尔茨海默病等神经退行性疾病的发生和进展中起着重要作用。因此，通过调节5HT信号通路可能成为一种治疗神经退行性疾病的方法。

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