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  4. Mouse 5-Hydroxytryptamine,5HT ELISA Kit

Mouse 5-Hydroxytryptamine,5HT ELISA Kit

文献引用 (10) 说明书下载
  • 中文名称：
    小鼠5羟色胺(5-HT)酶联免疫试剂盒
  • 货号：
    CSB-E08365m
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse 5HT ELISA Kit was designed for the quantitative measurement of Mouse 5HT protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 125 pg/mL.

  • 缩写：
    5HT
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    250 pg/mL-4000 pg/mL
  • 灵敏度：
    125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-89  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 84  
    Range % 80-88  
    1:800 Average % 97  
    Range % 92-101  
  • 回收率：
    The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.130 0.120 0.125    
    2000 0.243 0.241 0.242    
    1000 0.452 0.419 0.436    
    500 0.792 0.831 0.812    
    250 1.068 1.120 1.094    
    0 2.151 2.026 2.089    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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